Considerations To Know About hplc column packing
Considerations To Know About hplc column packing
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Huge selection of processes and stream route configurations available to layout your very own multi-step process.
In this type of chromatography, separation is predicated about the reversible interaction of proteins with ligands.
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For those who observe that there's a deterioration of peak condition coupled with boost in backpressure, it is usually recommended to reverse flush the column. Prior to reverse flushing it is necessary to ensure that your mobile phase or in-column solvent is miscible with all your cleaning solvents and that your move fees are not more than half The standard recommended move charge to the column. For in-depth procedures
Allows complete automation and integration with the VI, along with chromatography process administration with just one skid
Enables greater productivity than traditional chromatography, lessened buffer and resin volumes and lessen resin fees
Agilent-Qualified company industry experts can be found across the globe and retain an inventory of legitimate Agilent components. They’ll instantly diagnose your procedure onsite and complete any needed repairs—normally in a single take a look at.
A related method is a lot more compact and much easier to regulate. In this webinar, we give more info an summary on ways to configure the Resolute® BioSC.
And When you've got any more issues pertaining to how to best care for your column, operate an analysis, or anything else pertaining to work, Phenomenex provides a no cost specialized assistance by means of Stay Chat, which enables you to talk with Technical Professionals 24/7 worldwide. They have the ability to assist with Pretty much any challenge instantly.
Preferably, the temperature of your cell section and also the column needs to be saved constant during an Investigation.
Pumps differ in force capability, but their efficiency is calculated on their capacity to generate a consistent and reproducible volumetric circulation level.
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CIM® monoliths are available in several different chemistries and sizes starting from screening to industrial processing for higher yields and enhanced pace.
The quantitative parameters and equations which determine the extent of performance with the chromatographic procedure The parameters are mostly derived from two sets of chromatographic principle: plate theory (as A part of partition chromatography), and the rate principle of chromatography / Van Deemter equation.